An Integrated Analysis of Metabolites, Peptides, and Inflammation Biomarkers for Assessment of Preanalytical Variability of Human Plasma

Discrepancies in blood sample collection
and processing could have
a significant impact on levels of metabolites, peptides, and protein
biomarkers of inflammation in the blood; thus, sample quality control
is critical for successful biomarker identification and validation.
In this study, we analyzed the effects of several preanalytical processing
conditions, including different storage times and temperatures for
blood or plasma samples and different centrifugation forces on the
levels of metabolites, peptides, and inflammation biomarkers in human
plasma samples using ethylenediaminetetraacetic acid (EDTA) as an
anticoagulant. Temperature was found to be the major factor for metabolite
variation, and both time and temperature were identified as major
factors for peptide variation. For inflammation biomarkers, temperature
played different roles depending on the sample type (blood or plasma).
Low temperature affected inflammation biomarkers in blood, while room
temperature impacted inflammation biomarkers in plasma.