The type and relative distribution of the PP isolated RNAs by RNA-sequencing analysis in HeLa cells and dynamic investigation upon transcription inhibition by ActD treatment

Developing a psoralen probe (PP)-based method for RNA tagging and RNA-protein complex (RP-complex) enrichment, isolating of both coding and noncoding RNAs from HeLa cells was achieved by PP enrichment. Exploring the type and relative distribution of the PP isolated RNAs by RNA-sequencing analysis. And dynamic investigation of RNA upon transcription inhibition by ActD treatment. Overall design: The type and relative distribution of RNAs in HeLa cells by PP enrichment and TRIzol, RNA readcounts in HeLa cells treating with ActD