GSE10797 Expression Data

Transcriptomes of breast epithelium and stroma in normal reduction mammoplasty and invasive breast cancer patients. Breast tissue was collected at surgery from patients undergoing surgical resection for invasive breast cancer (n=28) and reduction mammoplasty (n=5). Tissue was cryopreserved in OCT and serial sections were cut using a cryostat. Sections were stained and epithelial and stromal cells were isolated by laser capture microdissection. Total RNA was isolated and target was prepared using NuGen Ribo-SPIA Ovation Technology. Gene expression was measured using Affymetrix U133A 2.0 GeneChips. Gene expression differences were evaluated using a linear model of the Robust Multichip Average gene expression statistic, a model that accounted for cell type (E and S), patient diagnosis (C and N), as well as cell type-diagnosis interactions. Data were examined to determine false discovery rate and were filtered for fold changes in gene expression and p-value. This data set was originally published on Apr 3, 2008 on GEO. It can be viewed here: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE10797