Extragenous ILC2s infiltration into the brain after ischemic stroke

Group 2 innate lymphoid cells (ILC2s) are key players in immune regulation and tissue repair in barrier immunity, but they are rarely seen in the brain. We investigated whether ILC2s in the peripheral circulation during the acute phase could infiltrate the brain parenchyma. We first transfer ILC2s intravenously into the MCAO mice and performed RNA sequencing of ILC2s isolated from the blood and the brain at different time points after MCAO. We collected the transferred ILC2s from the blood at day 1 (bld_1d, n=6) and day 5 (bld_5d, n=6), also from the brain at day 5 after MCAO (br_5d, n=6) for RNA-seq