Comparative effects of two different aluminum nanoparticle formulations on the histological and physiological aspects of Drosophila melanogaster (Diptera: Drosophilidae) as a model

Recycling aluminum, which is widely utilized in industries such as packaging, poses a significant challenge in selecting the most efficient method that retains its properties while minimizing environmental impact. This study investigates the potential adverse effects of aluminum oxide nanoparticles (Al2O3 NPs) synthesized from disposable aluminum foil waste using two different recycling methods: conventional grinding (CG) and ball milling (BM). This study employs Drosophila melanogaster as a well-established in vivo model organism to determine which recycling technique yields nanoparticles (NPs) that are less harm to non-targets. To assess this, relevant concentration of Al2O3 NPs formulations was added into the food media. Seven days post feeding, alterations of adult’s midgut were analyzed at physiological and ultrastructure levels. The results showed that Al2O3 NPs had significant effects on digestive enzymes, regardless of how they are synthesized. In contrast to chitinase and prote..., This dataset originates from a study investigating the biological effects of aluminum oxide nanoparticles (Al₂O₃ NPs) synthesized from recycled aluminum foil waste. The synthesis involved dissolving cleaned and shredded aluminum foil in hydrochloric acid, followed by precipitation using sodium carbonate to form aluminum oxide. The resulting material was washed, dried, calcined at 800°C, and then ground using two methods—conventional grinding (CG) and ball milling (BM)—to obtain nanoscale particles. These formulations were characterized using FTIR, XRD, SEM, and TEM.
Biological assays were conducted on Drosophila melanogaster (wild-type Canton S) to assess the physiological effects of CG and BM Al₂O₃ NPs. Flies were exposed to nanoparticle-supplemented food media, and multiple endpoints were evaluated: food intake using the capillary feeding (CAFE) assay, defecation rate via fecal pellet count, digestive enzyme activities in dissected midguts (seven enzymes assessed using colorimetric ki..., # Comparative effects of two different aluminum nanoparticle formulations on the histological and physiological aspects of Drosophila melanogaster (Diptera: Drosophilidae) as a model
Authors: Hanaa Elbrense, Marwa N. El-Nahass, Samar El-Kholy

Corresponding author: Samar El Kholy ([e-mail]) ORCID number: 0000-0003-2816-7243

Dataset DOI:10.5061/dryad.wwpzgmsxf

## Description of the data and file structure

The present study investigates the potential adverse effects of aluminum oxide nanoparticles (Al2O3 NPs) synthesized from disposable aluminum foil waste using two different recycling methods: conventional grinding (CG) and ball milling (BM). This study employs Drosophila melanogaster as in vivo model organism to determine which recycling technique yields nanoparticles (NPs) that are less harm to non-targets. To assess this, relevant concentration of Al2O3 NPs formulations was added into the food media. Seven days post feeding, alterations of adultís midgut w...,