RNA-sequencing of forward programmed hepatocytes and fetal liver samples

The purpose of this experiment was to compare the transcriptome of FoP-Heps, with undifferentiated hiPSCs, HLCs generated by direct differentiation, and primary samples (adult and fetal). FoP-Heps where generated in vitro from hESCs by forward programming (FoP) using a combination of 4 transcription factors (HNF1A, FOXA3, HNF6, RORc). Human fetal liver samples where obtained from first trimester embryos.