The intestine transcriptome analysis of hybrid yellow catfish (Tachysurus fulvidraco♀ × P. vachellii♂) under transport stress

The transport of live fish is a routine operation in aquaculture. However, the effects of transport stress on intestines gene expression profile is poorly understood. The results from the transcriptome analysis revealed that most differentially expression genes (DEGs) were enriched in immune and metabolic related signaling pathways. Toll like receptors is a potential pathway in immune response induced by transport stress. Changes in genes related to lipid metabolism may be related to the increased metabolic expenditure for the normal body functions in a response to transport stress. In addition, changes in gene expression in the control, transport, and recovery groups were revealed that transport-induced local immune damage is reversible while metabolic disorder has hysteresis. These results provide new perspectives on immune and metabolic response in yellow catfish against transport stress and theoretical support for future optimization of their transportation. In this study, hybrid yellow catfish ((Tachysurus fulvidraco♀ * P. vachellii♂) were subjected to simulate the transport (0h, 2h, 4h, 8h, 16h) and recover 96h, and intestinal biochemical and transcriptome analyses were performed to broaden our understanding of fish under transport stress.