S2_Fig

S2 Fig. Exogenous effect of ascorbate and its analogue on the stress sensitivity of ara2Δ yeast cells. (A) Monitoring of the effect was performed by spotting assay. Yeast cells pretreated with 10 mM AsA and 10 mM IAA for 1 h were exposed to 20 mM H2O2 for 1 h. Five microliters of diluted solutions were spotted onto YPD agar plates. Exogenous AsA- and IAA-treated cells showed better survival compared to cells under normal conditions, suggesting that their concentrations were sufficient for detoxifying endogenous and exogenous ROS and enhanced cell proliferation. WT, yeast cells transformed an empty vector; TC, OsMDHAR-expressing yeast cells. (B) Stress sensitivity of ara2Δ yeast cells to oxidative stress. Yeast cells were treated with abiotic stressors for 1 h with shaking and spotted onto YPD agar plates. For heat shock, yeast cells were incubated for 5 min at 55ºC, and then spotted as described above. BY, wild-type yeast cells without an empty vector; ara2Δ, yeast cells in which the ARA2 gene had been deleted.