5PSeq Explorer Data Freeze

This data freeze accompanies 5PSeq Explorer and it contains 775 5PSeq datasets compiled from 18 published studies interrogating various aspects of biology at the interface of mRNA decay and translation, including both genetic and environmental perturbations. To enable comparability across conditions and cross-species we processed all data uniformly (see Methods). The generated database consists of: (1) metadata, (2) raw processed counts files of amino acid, codon, frame preferences, metagene counts around start and termination sites, additional count files as documented on the Fivepseq package output documentation, and (3) RNA composition.

For Eukaryotes, the collection consists of 378 yeast samples (Ascomycota phylum) with an overrepresentation for Saccharomyces cerevisiae (n= 340). For bacteria, we provide 397 samples across Actinomycetota, Bacillota, Bacteroides, Bacteroidota, Proteobacteria, Pseudomonadota and Verrucomicrobiota phyla with an overreprepresentation for Bacillus subtilis (n= 67) and Aggregatibacter actinomycetemcomitans (n=59).