Dataset for "Fluorescent Styrene Maleic Acid Copolymers to Facilitate Membrane Protein Studies in Lipid Nanodiscs"

The purpose for obtaining this data was to characterise fluorescent styrene maleic acid copolymers synthesised via RAFT copolymerisation with styrene-analogous fluorophores. Copolymer behaviours were minimally affected and were able to spontaneously form lipid nanodiscs (SMALPs) upon incubation with DMPC vesicles. The potential usefulness of these novel copolymers to membrane protein (MP) research was also assessed. FRET was found to occur between the copolymer annulus and an encapsulated model MP, gramicidin, indicative of proximity. Further, aggregation caused quenching phenomena were used to monitor the transition from aggregated copolymer to nanodisc annulus during SMALP self assembly. Here, both the raw and processed data used in the publication are presented as .xls, .txt, and .pdf files, as indexed in the dataset.

本数据集的获取旨在对通过RAFT共聚反应合成的荧光苯乙烯马来酸酐共聚物进行表征（合成过程中使用了苯乙烯类似荧光团）。共聚物的行为受影响极小，并且能够在与DMPC脂质体制剂孵育后自发形成脂质纳米盘（SMALPs）。此外，还对这些新型共聚物在膜蛋白（MP）研究中的潜在应用价值进行了评估。研究发现，共聚物环与封装的模型MP（链霉素）之间存在FRET现象，这表明了两者之间的邻近性。此外，通过聚集引起的猝灭现象被用于监测SMALP自组装过程中从聚集的共聚物到纳米盘环的转变。在此，出版物中使用的原始数据和处理后的数据均以.xls、.txt和.pdf文件格式提供，并在数据集中进行了索引。