Deep-sequencing of small RNAs from barley shoot and root growing in low-Pi and control condition.. Deep-sequencing of small RNAs from barley shoot and root growing in low-Pi and control condition.

RNA was isolated from 23 old barley plants (shoots and roots), line Rolap. We used a modified method that allows for enrichment of small RNAs. Libraries were prepared using TruSeq Small RNA Library Preparation Kit (Illumina), followed by sequencing of NGS libraries. Overall design: Total RNA isolation, libraries preparation, single-reads (50 cycles) sequencing, Illumina technology, HiSeq 4000, sequencing performed at Fasteris SA (Switzerland).