A label-free quantitative proteomic analysis of mouse neutrophil in huazhong agricultural

Streptococcus suis (S. suis) ranks among the five most important porcine pathogens worldwide and occasionally threatens human health, especially in people who come into close contact with pigs or pork products. An S. suis infection induces the formation of neutrophil extracellular trap (NET) in vitro and in vivo, and the NET structure plays an essential role in S. suis clearance. However, the mechanism of NET induction remains to be elucidated. In the present study, we used a label-free quantitative proteomic analysis of mouse NET formation induced by S. suis or phorbol myristate acetate (PMA), a robust NET inducer. We found that more than 50% of the differentially expressed proteins of neutrophils infected by S. .