Characterisation by Next generation Capture-C of Pax6 locus in a mouse cell line with alpha amanatin treatment to inhibit transcription

NG-Capture-C profiling of chromatin interactions (using DpnII 3C and Oligo nucleotide sequence capture followed by Next generation sequencing) of enhancers, promoters and CTCF/Rad21 binding sites throughout a ~600kb region of the mouse Pax6 locus in β-TC3 cells treated with alpha amanitin. β-TC3 cell are a mouse pancreatic β cell derived from mouse insulinoma. β-TC3 mouse cells were analysed after being treated either with alpha amanitin or with mock treatment (PBS alone). Two separate panels of capture oligos were used (denoted the CTCF pool and the PROM pool), with two biological replicates generated in each case.