Catalysis of Tos-Gly-Pro-Arg-p-nitroanilide by α-thrombin in the presence of an anticoagulant produced by D. andersoni (dataset formatted for analysis by interferENZY)

Main description

This dataset depicts the catalysis of the chromogenic substrate Tos-Gly-Pro-Arg-p-nitroanilide by α-thrombin in the presence of an anticoagulant produced by Dermacentor andersoni, for fixed concentration of enzyme (and modulator) and variation of concentration of initial substrate. It was originally documented in Biophysical Chemistry 252 (2019) 106193 (https://doi.org/10.1016/j.bpc.2019.106193), and PNAS 116 (28) 13873-13878 (https://doi.org/10.1073/pnas.1905177116), and then used as a study case for the webserver interferENZY (a web-based tool for enzymatic assay validation and standardized kinetic analysis; visit https://interferenzy.i3s.up.pt for more information). To this end, it was converted to the format here presented: tab-separated *.txt input required for interferENZY analysis.

Dataset organization

Line 1: Tab-separated initial concentrations of substrate Tos-Gly-Pro-Arg-p-nitroanilide in micromolar (µM) concentration

Line 2: Concentration of enzyme (0.15 nM)

Line 3: Units of time

Line 4: Units of concentration for substrate values and measurements

Line 5: Dataset name

Line 6 and downwards: Tab-separated column-pairs of the progress curves (time,Product) corresponding to the indicated values of initial concentrations of substrate in line 1

Contact information:

Maria Filipa Pinto ([e-mail])
Pedro M. Martins ([e-mail])

i3S – Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal. Telephone number: [phone]