miRNA sequencing detecting miRNA cargo from PRP derived exosomes isolated from young donors and old donors. miRNA sequencing detecting miRNA cargo from PRP derived exosomes isolated from young donors and old donors

We detected and compared the miRNAs contained in Y-PRP-exos or O-PRP-exos. The results showed that most miRNAs (38) can be identified in both Y-PRP-exos and O-PRP-exos. In addition, hsa-let-7f-5p, hsa-miR-16-5p and hsa-miR-126-3p were top 3 enriched miRNAs in PRP-exos of all donors. These results indicated the heterogeneity of miRNAs in PRP-exos was small, regardless of age. However, the abundance of miRNAs in Y-PRP-exos and O-PRP-exos was quite different, especially in high-ranking miRNAs. The expression of hsa-miR-16-5p and has-let-7f-5p in O-PRP-exos in two old donors was only approximately 1/4-1/2 of that in Y-PRP-exos in two young donors Overall design: To investigate the miRNAs in PRP-exosomes that could regulate adipogenesis and proliferation of Pdgfrα+ progenitors and fatty infiltration in muscle injury, we isolated PRP exosomes derived from two young donors and two old donors, respectively, and compared the enriched miRNAs.