RNA-seq profiling of in vitro activated naïve B cells reveals differences in B-cell activation and mismatch repair machinery in common variable immune deficiency (CVID)

To investigate the transcriptional dfferences during B-cell activation in healthy donors and patients with CVID we sorted naïve B cells from the two groups and activated with full BCR-engagement. We then performed differential expression analysis on the seuqneces and we also investigated what pathway changes that we could detect between healthy donors and CVID patients. Comparative gene expression profiling analysis of RNA-seq data derived from in-vitro activated naïve B cells of health control and patients with common variable immune deficiency (CVID)