Epigenetic patterns with aging and exercise are associated with indicators of healthspan in humans

Genome wise DNA methylation profiling of peripheral blood mononuclear cells (PBMCs) obtained from younger sedentary (Y-SED), older sedentary (O-SED) and older aerobically exercise trained (O-Ex) human subjects. The Illumina 450K methylation beadchip array was used to obtain DNA methylation profiles across approximately 450,000 CpG dinucleotide methylation loci in DNA isolated from PBMCs. Samples include 12 Y-SED subjects, 15 O-SED subjects and 11 O-Ex subjects. DNA methylation was assessed in peripheral blood mononuclear cells (PBMCs) of 12 young-sedentary (Y-SED), 15 older-sedentary (O-SED) and 11 older exercise trained (O-Ex) individuals using the Illumina HumanMethylation 450K array.