Z. Xu, H. Ogawa, P. Vagnarelli, J.H. Bergmann, D.F. Hudson, S. Ruchard, T. Fukagawa, W.C. Earnshaw, K. Samejima (2011) CIL:12217, Gallus gallus gallus, memory B cell. CIL. Dataset
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Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Prometaphase in INCENP-OFF cells expressing TrAP-INCENP WT. Histone H3 modification H3-S10ph shown in green. DAPI (DNA) is blue. See Supplementary Fig 3E panels 5-7 in Supplementary data for Xu et al. 2009 J Cell Biol 187, 637-653.
DT40鸡B淋巴细胞系细胞在有丝分裂阶段。使用Deltavision系统,通过100x/1.4NA计划阿波目镜捕捉到的宽场免疫荧光图像的Z系列。图像经约束迭代算法(Applied Precision)反卷积处理。展示的是中央的Z切片。在INCENP-OFF细胞中表达TrAP-INCENP WT的细胞处于前期。绿色显示组蛋白H3的修饰H3-S10ph。DAPI(DNA)呈蓝色。详见补充数据中Xu等人2009年《细胞生物学杂志》第187卷第637-653页的补充图3E面板5-7。
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CIL



