Characterization of Global DNA Methylome in Mild Cognitive Impairment and Alzheimer's Disease

Genome-wide profiling of DNA methylation in blood leukocytes from Chinese patients with mild cognitive impairment (MCI) and Alzheimer’s disease (AD). The Illumina Infinium MethylationEPIC BeadChip array (850K chip) was used to detect DNA methylation profiles throughout approximately 850,000 CpG sites in peripheral blood white cells of MCI- and AD-affected Chinese patients, as well as cognitively healthy controls. All samples included 20 Chinese patients with MCI, 20 Chinese patients with AD, and 20 cognitively healthy controls. Genomic DNA was converted by bisulphite, and subsequently hybridised to the Illumina Infinium MethylationEPIC BeadChip.