Absolute protein abundances (μg/g_protein) in R. toruloides batch cultivations on three different carbon substrates–glucose (G), xylose (X) and acetate (A)–during exponential growth (exp) and nitrogen limitation (Nlim) phases.

Concentrations are calculated using total protein amount (TPA) quantification method. Pairs having adjusted p-value |1| of average of duplicate conditions were considered significantly differentially expressed. P value was adjusted for multiple comparisons (n = 3100) using Benjamini & Hochberg method [58]. Protein abundances were filtered by excluding instances, where standard deviation exceeds mean value of two replicates. Normalization refers to 80% recovery of the sample injected applied to the sum of intensities. (XLSX)