SCR106 splicing factor modulates abiotic stress responses by maintaining the RNA splicing in rice. SCR106 splicing factor modulates abiotic stress responses by maintaining the RNA splicing in rice

We investigated the function of the plant-specific SR protein SC106 in pre-mRNA splicing regulation and abiotic stress responses in rice. The loss-of-function mutant, SC106, showed increased sensitivity to salt. We performed genome-wide analyses of gene expression and splicing in seedlings subjected to salt stress and identified multiple splice isoforms from stress-responsive genes dependent on SC106. Overall design: 7-day-old seedlings of sc106 mutant, and WT germinated on 125mM NaCl and mock conditions. . The RNA-seq libraries were constructed using TruSeq mRNA stranded kit following the standard protocol and sequenced on the Nova-seq platform to generate high-quality paired-end reads.