16S rDNA amplicon sequencing

16S rDNA amplicon sequencing of soil bacterial comunities isolated from soils on which plants were grown and iteratively exposed to a foliar pathogen. The global aim was to follow the bacterial dynamic during different soil conditionings (conductive, suppressive, control or non-conditioned) by Arabidopsis thaliana in response to the foliar pathogen Pseudomonas syringae DC3000 (Pst). On mesocosms dedicated to soil conditioning, 28 days old plants were infiltrated with Pst while other (non-conditioned) were mock-infiltrated with a saline buffer. Seven days after inoculation (d.p.i), plants were selected accordingly to the expected soil conditioning. Experiment was conducted for 10 iterations with each iteration comprising the same 3 steps: (i) plant growing (during 28 days), (ii) Pst or mock inoculation and (iii) mesocosm selection (at 7 d.p.i). Selected mesocosms were kept accordingly to the excepted soil conditioning and used to prepare the mesocosms of the following iteration. After the selection, soil was retrieved and environmental DNA extracted before 16S rDNA amplicon sequencing.