Whole genome microarray gene expression profiling of peripheral blood mononuclear cells (PBMC) isolated from old and middle-aged, voluntary human research participants

With increasing age of the global population, age-associated diseases such as cardiovascular diseases, cancer or diabetes are on the rise in many countries. To identify cellular processes and markers of aging in humans, this study performed comparative whole genome microarray gene expression profiling of peripheral blood mononuclear cells (PBMC) isolated from two age groups, i.e., an old age group (age: 75-89 years) and a younger, middle-aged group (age: 35-50 years). Total RNA was isolated from the PBMC and subjected to whole genome microarray gene expression profiling. The whole genome gene expression data of human PBMC reveal age-associated changes in transcript levels and contribute to a better understanding of biological processes underlying aging and the pathogenesis of age-associated diseases. The study was performed with total RNA isolated from peripheral blood mononuclear cells (PBMC) of two age groups of voluntary human research participants. The first age group encompasses old human research participants (age: 75-89 years; n=5), and the second group are middle-aged human research participants aged 35-50 years (n=4).