Phenotypic and transcriptomic impact of expressing mammalian TET2 in the Drosophila melanogaster model

To examine the Ten-Eleven Translocation (TET) proteins and their role in tumorigenesis in hemocytes and heads in Drosophila melanogaster. To identify the transcriptomic profile of wild type mTET2 versus mTET2 mutants (catalytic versus non-catalytic) to investigate TET2 role in normal central nervous system (CNS) function and innate immunity. • For RNA-Seq experiment, the following four Drosophila Melanogaster groups were used: mTET2-Wt (flies expressing wild type mammalian TET2), mTet2-R1261C (flies expressing mammalian TET2 with an Arginine to Cysteine mutation; R1261C), mTET2-R43G (flies expressing mammalian TET2 with an Arginine to Glycine mutation; R43G), and W1118 (wild type flies; control). 12 RNA samples from heads of these 4 groups of flies (3 biological replicates for each group) were used for RNA-Seq analysis.