RNA Sequencing Analysis of macrophage and CD4+T cells from Wild Type and Gsdmd-/- mice

We identified that GSDMD was required for the pathogenesis of EAE, and GSDMD deficiency in peripheral myeloid cells impaired the infiltration of immune cells into the CNS , leading to the suppression of neuroinflammation and demyelination.The purpose of this experiment was to identify how GSDMD induces EAE. Overall design: Macrophage and CD4+T cells mRNA profiles of wild type (WT) and Gsdmd-/- mice with experimental autoimmune encephalomyelitis were generated by deep sequencing, in triplicate, using BGISEQ-500