iMGMC - integrated Mouse Gut Metagenomic Catalog

Creation of an new mouse gut gene catalog with special features:

more diverse samples from different studies (12 Vendors incl. wild mice and various gut locations)

clustering-free approach: all-in-one assembly, keeping track of each ORF to contigs to bins

higher taxonomic resolution and more accuracy by using contigs for annotation

16S rRNA gene integration via linkage to bins

expansion by 20,927 MAGs from sample-wise assembly of 871 mouse gut metagenomic samples, representing 1,296 species

Code used: https://github.com/tillrobin/iMGMC

The vast complexity of host-associated microbial ecosystems requires host-specific reference catalogs to survey the functions and diversity of these communities. We generated a comprehensive resource, the integrated mouse gut metagenome catalog (iMGMC), comprising 4.6 million unique genes and 660 metagenome-assembled genomes (MAGs) with many of them (485 MAGs, 73%) linked to reconstructed full-length 16S rRNA gene sequences. iMGMC enables unprecedented coverage and taxonomic resolution of the mouse gut microbiota, i.e. more than 92% of MAGs lack species-level representatives in public repositories (<95% ANI match). The integration of MAGs and 16S rRNA gene data allows a more accurate prediction of functional profiles of communities than based on 16S rRNA amplicons alone. Accompanying iMGMC we provide a set of MAGs representing 1,296 gut bacteria obtained through complementary assembly strategies. We envision that integrated resources such as iMGMC together with MAG collections will enhance the resolution of numerous existing and future sequencing-based studies.

Genecatalog:

Description    Size   Filename
Catalog ORF sequences    1 GB    iMGMC-GeneID.fasta.gz
Full assembly contigs    1.3 GB    iMGMC-ConitgID.fasta.gz
Mapping File (GeneID->ContigID->BinID)    30 MB    iMGMC-map-Gene-Contig-Bin.tab.gz
Taxonomic annotations    40 MB    iMGMC_map_taxonomy.tar.gz
Functional annotations    36 MB    iMGMC_map_functionality.tar.gz
16S rRNA sequences    2 MB    iMGMC-16SrRNAgenes.fasta

Metagenome-assembled genomes (MAGs) :

Description    Size    Filename
integrated MAGs    0.5 GB    iMGMC_MAGs.tar.gz
representave mMAGs (n=1296)    1 GB    iMGMC-mMAGs-dereplicated_genomes.tar.gz
representave hqMAGs (n=830)    0.7 GB    iMGMC-hqMAGs-dereplicated_genomes.tar.gz
all mMAGs (n=20,927)    15 GB    iMGMC-mMAGs.tar.gz
Annotations by CheckM, dRep-Clustering, GTDB-Tk    2 MB    MAG-annotation_CheckM_dRep_GTDB-Tk.tar.gz
Functional annotations (hqMAGs by eggNOG mapper v2)    187 MB    hqMAGs.emapper.annotations.gz