A sputum bioassay for airway eosinophilia using an eosinophil peroxidase aptamer

We genearted a DNA aptamer against EPX via SELEX protocol using a random DNA library and developed an assay to monitor EPX in patients spitum samples After 15 rounds of selection, the aptamer sequences were obtained by high throughput sequencing Overall design: A random DNA library was obtained by chemical synthesis, the target EPX was covalently immobilized on magnetic beads, then in vitro selection was caried out. After selection, we did deep sequencing with the Illumina MiSEQ instrument which gave us the fastq files. These files were analyzed and processed by Oracle software to sort out our top scored sequences. Top 5 sequences were used in our project to evaluate binding and developing assay.