Comparison of ITS and RIF markers for three genera of Enterobacteriaceae.

RIF and ITS marker characteristics for a set of 96 strains from the PBC collection. Sequence contigs are assembled from read pairs. *- Computed using a single representative for each unique sequence. a- Eight Pectobacterium strains and one Pantoea strain failed to yield either ITS or RIF amplicons after multiple attempts to produce an amplicon from reisolated DNA (Supplemental Table S2). b- Pantoea strains were not sequenced with ITS. c- 15 strains were amplified at 61°C and 3 strains at 51°C. d- 2 strains were amplified at 61°C and 4 strains at 51°C. e- 2 strains were amplified at 61°C and 2 strains at 51°C.