Vibrio natriegens has recently gained attention as a novel fast-growing bacterium in synthetic biology applications. Currently, a limited set of genetic elements optimised for Escherichia coli are use
Asterisks (*) indicate previously existing Biobricks. “Registry” refers to the Registry reference, whereas “GenBank” refers to the GenBank accession number.
Additional file 2: Table S1. Growth Rates. Table S2. Plasmids used in this study. Table S3. Strains used in this study. Table S4. Primers used in this study. Table S5. Sequences of Promoters and Repor
Ribosome binding sites and stop codons provide signals for where translation should start and stop, and secondary structures in messenger RNAs can stall ribosomes and induce frameshifting. We develop