RNA-seq in NT, CAP-D3, and CAP-H2 expressing cells treated with DMSO or menadione

The goal of this experiment was to identify transcripts that are differentially expressed in CAP-D3 and CAP-H2 depleted cells with and without exposure to menadione Overall design: RNA was isoalted from HT cells expressing Non-Target, CAP-D3, and CAP-H2 shRNA, post DMSO or menadione treatment. cDNA libraries (50 bp, paired end) were made at the University of Chicago Genomics Core and sequenced on an Illumina HiSeq2500, according to standard protocols.