Inhibition of viral replication by XCR1- and XCR1+ cDC1

In order to analyze whether type I and III IFN secreted by XCR1- and XCR1+ cDC1 inhibit viral replication, sorted XCR1- and XCR1+ cDC1 were stimulated with pIC for 12h. Supernatants were harvested and used to induce an antiviral state in U2OS cells. Concentration of IFN was determined in the supernatants using the LEGENDplex Human Type 1/2/3 Interferon Panel (5-plex) with V-bottom Plate.
Conclusion:
XCR1+ cDC1 excel in the secretion of type I and III IFN, which correlates with the superior induction of an antiviral state in epithelial cells.
Notes:
Published in: Proceedings of the National Academy of Sciences (PNAS), 2023 Title: XCR1 expression distinguishes human conventional dendritic cell type 1 with full effector function from their immediate precursors. Authors: Lukas Heger, Lukas Hatscher, Chunguang Liang, Christian H. K. Lehmann, Lukas Amon, Jennifer J. Lühr, Tomasz Kaszubowski, Rayk Nzirorera, Niels Schaft, Jan Dörrie, Pascal Irrgang, Matthias Tenbusch, Meik Kunz, Eileen Socher, Stella E. Autenrieth, Ariawan Purbojo, Horia Sirbu, Arndt Hartmann, Christoph Alexiou, Robert Cesnjevar, and Diana Dudziak doi: 10.1073/pnas.2300343120 Standard curve using lyophylized proteins.