Transcriptional profiling of Regulatory T-cells isolated from neonatal skin and skin draining lymph nodes (SDLNs)

Purpose: The goals of this study were to identify preferential gene expression signatures that are unique to Tregs in neonatal skin relative to peripheral Tregs Methods: Tregs from telogen skin and SDLNs were purified by cell sorting (using the Treg GFP reporter mouse line Foxp3-DTR/GFP) to generate mRNA transcription profiles. Results: Transcriptional profiling revealed a unique neonatal skin Treg signature relative to SDLN Tregs Conclusion: Our study represents the first detailed analysis of the neonatal skin Treg transcriptome. mRNA profiles of skin and SDLN Tregs isolated from 13 day old Foxp3-DTR/GFP mice.