脊髓投射神经元单细胞转录组图谱

脊髓是躯体感觉信息加工处理的重要初级中枢，其中脊髓投射神经元是连接脊髓和大脑的桥梁，负责将不同形式的躯体感觉信息从脊髓传递到大脑，是躯体感觉系统中的关键节点。我们结合了单细胞转录组测序和MERFISH空间转录组技术在分子水平对脊髓投射神经元进行系统性分类，全面地绘制了包含15个亚群，9,759个脊髓投射神经元的单细胞转录组图谱，并鉴定各亚群在脊髓内的分布模式。此外，我们利用活性依赖的单细胞转录组测序技术，系统地鉴定了被痛觉和痒觉激活的脊髓投射神经元亚群。

The spinal cord is a critical primary central hub for somatosensory information processing. Spinal projection neurons act as the bridge connecting the spinal cord and the brain, responsible for transmitting diverse forms of somatosensory information from the spinal cord to the brain, and serve as key nodes in the somatosensory system. We combined single-cell RNA sequencing and MERFISH spatial transcriptomics technologies to conduct a systematic classification of spinal projection neurons at the molecular level, comprehensively constructing a single-cell transcriptomic atlas encompassing 15 subtypes and 9,759 spinal projection neurons, and characterizing the in-spinal distribution patterns of each subtype. Additionally, we utilized activity-dependent single-cell RNA sequencing technology to systematically identify spinal projection neuron subtypes activated by nociceptive and pruriceptive stimuli.

搜集汇总

背景与挑战

背景概述

该数据集通过单细胞RNA测序和空间转录组技术，绘制了9,759个脊髓投射神经元的转录组图谱，识别出15个亚型并揭示了其空间分布。同时，利用活动依赖性方法分析了伤害性和瘙痒性刺激激活的亚型，首次多角度表征了抑制性神经元。