Aggregated frequencies of transcription initiations observed in FANTOM5 CAGE data on GRCm38, including alignments with low mapping qualities

Overview

Aligned reads of the FANTOM5 CAGE data have been used after filtering (ones with mapping quality less than 20 or percent identity less than 85% were discarded) for general purpose, resulting in the data set consisting of only the reads aligned with confidence. The filtering process made possible to interpret the data without ambiguity, however it also limited interpretation of paralogous or duplicated regions within the genome. Here all of the 5'-ends of the CAGE read alignments, including the ones with low mapping quality, were counted. The counts in the individual profiles were aggregated and summed up. 

This data set is produced for mouse, in the same way to the one for human data set http://doi.org/10.5281/zenodo.1410835

Data files

The resulting data files are formatted as bigWig (https://genome.ucsc.edu/FAQ/FAQformat.html#format6.1). '*.fwd.bw' and '*.rev.bw' represent forward and reverse strand on the genome, respectively. 

Methods

The BAM files under https://fantom.gsc.riken.jp/5/datafiles/reprocessed/mm10_v7/basic/ were subjected to 5'-end counting by bedtools v2.27.1 (https://github.com/arq5x/bedtools2), followed by conversion into bigWig with jksrc v366 (http://hgdownload.cse.ucsc.edu/admin/).