Analysis of Human Membrane Protein Complexes by Using Formaldehyde

Membrane proteins are essential to many biological processes and account for two-thirds of the successful drug targets. Studying the membrane-protein complexes assembly will help to understand their biological functions comprehensively. We applied an approach that combines cross-linking mass spectrometry and protein correlation profiling to analysis the endogenous human membrane protein complexes. Finally, we constructed a network of 312 complexes involves 31,095 high-confident interactions with 2,333 proteins. These protein complexes are linked to core biological processes such as translation, intracellular transport, metabolic, and catabolic. They are also related to more than one hundred of human diseases, especially Alzheimer's disease, Carcinoma, and Endocarditis. Strikingly