Kinetic constants of the inactivation process of native VP and its mutated variants.

(ki, first-order inactivation rate constant; KI, H2O2:VP molar ratio resulting in the half maximum inactivation rate; kapp, apparent second-order oxidative inactivation rate constant; and n, Hill coefficient).Reactions at 25°C in 0.1 M tartrate, pH 5, using 0.01 μM VP, final concentration, and increasing stoichiometric excesses of H2O2. Means and 95% confidence limits.an.d. Not determined because saturation was not reached.Kinetic constants of the inactivation process of native VP and its mutated variants.