Transcriptome of gemcitabine-treated 4T1 mouse tumors

This study aims to investigate the transcriptomic changes in 4T1 mouse breast cancer tumors following treatment with the chemotherapeutic drug gemcitabine. The 4T1 cells were implanted into BALB/c mice to establish a tumor-bearing model. After tumor formation, the mice were treated with gemcitabine. Tumor tissues were then collected from both the gemcitabine-treated group and a control group. RNA sequencing was performed to identify differentially expressed genes and signaling pathways affected by gemcitabine. The goal is to elucidate the molecular mechanisms of gemcitabine's anti-tumor effects and potential resistance mechanisms in this aggressive breast cancer model.