Systematics and evolutionary history of raft and nursery-web spiders (Araneae: Dolomedidae and Pisauridae)

Pisauridae are a global and heterogeneous assemblage of spider genera with diverse morphologies and lifestyles. So far, the monophyly of Pisauridae and the inclusion of fishing spiders (Dolomedes) in this family have not been thoroughly tested. Here, we amend the systematics and classification of these lineages within a UCE phylogenomic framework and through a detailed morphological reappraisal. For estimations of their evolutionary age, we perform and compare outcomes from two divergence estimation approaches, an a posteriori likelihood, and an a priori Bayesian. Phylogenies reject the monophyly of both Pisauridae and Dolomedes: 1) Focal Clade I groups true Pisauridae genera including Pisaura; 2) Focal Clade II contains Blandinia and is sister to Trechaleidae and Lycosidae; 3) Focal Clade III groups Dolomedes, Megadolomedes, and Ornodolomedes, and is sister to Blandinia, Trechaleidae, and Lycosidae. We therefore propose to delimit Pisauridae by removing Dolomedidae rank resurrected (in..., , , # Systematics and evolutionary history of raft and nursery-web spiders (Araneae: Dolomedidae and Pisauridae): Open Source Data

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We have submitted our raw UCE submatrix (**UCE_submatrix.zip**), configuration and input files for MCMCtree divergence time estimation (**MCMCtree.zip**), comparisons between MCMCtree and RelTime calibration methods (**Divergence_time_compare.zip**), and the publication's supporting information (**Supporting_information.zip**).

## Descriptions

### **UCE\_submatrix**

The folder contains the UCE matrix, in nexus format, used to estimate the divergence time of the taxon in question. This matrix was aligned from 300 UCE loci randomly subsampled from the full UCE matrix in our self-archived project paper: Yu, Cheng *et al*., 2024 ([https://doi.org/10.1101/2024.08.23.609317](https://doi.org/10.1101/2024.08.23.609317))

### **MCMCtree**

This folder contains two subfolders (listed below) containing an R script and configuration files to run MCMCtree divergence...