Microglia coordinate the anti-tumor immune response to breast cancer brain metastasis [MITRG 10X data]

We used single-cell RNA-sequencing to investigate the role of myeloid cells in a humanized mouse model of breast cancer brain metastasis (BCBM). We generated brain metastases with a cardiac-injection of the human triple-negative breast cancer cell line MDA-MB-231BR2 (231BR) into MITRG mice and allowed tumors to grow in the brain for 25 days. We then took all human cells (includes myeloid cells and 231BR) from these mice verses control MITRG brains (only myeloid cells) to find transcriptional changes associated with BCBM. Single-cell RNA-Seq of human iPSC-derived myeloid cells and MDA-MB-231BR2 from the brains of MITRG mice with and without breast cancer brain metastases. Please note that, as both BAM and FASTQ are necessary to interpret the data, duplicated sample records are created to link two different raw data type (i.e. BAM containing cDNA reads and fastq containing a set of DNA barcodes from the MULTI-seq element)