Accurate prediction of RNA translation with a deep learning architecture

Validation of motifs around strong TTS. Known readthrough stop codon from VDR and their mutations (without change of amino acids) were tested for translation readthrough. No_stop: stop codon of VDR (TGA) was mutated to CGA; strong TTS1: stop codon of VDR was mutated to a strong TTS by changing the upstream 27nt and downstream 12nt sequences; strong TTS2: stop codon of VDR was mutated to a strong TTS by changing the upstream 27nt sequence; weak TTS: stop codon of VDR was mutated to a weak TTS by changing the upstream 27nt.