CRISPR/Cas9 mediated deletion of the adenosine A2AR enhances CAR T cell efficacy [human_CRISPR_A2AKO_RNA-seq]

Investigation of the transcriptional profile of stimulated HUMAN CRISPR/Cas9 MOCK and A2ARKO a-lewisY CAR T cells in the presence/absence of NECA Overall design: Human anti-Lewis Y CAR T cells were generated from healthy donor peripheral blood mononuclear cells (PBMC) and then modified with CRISPR/Cas9 and a sgRNA targeting either human A2AR or a non-targeting control. CAR T cells were then cultured for another 6 days. MOCK or A2AKO CAR T cells were stimulated with plate bound anti-Lewis-Y idiotypic (anti-CAR) antibody in the presence or absence of NECA (10 µM) for 8 hours. Cells were analyzed with 3' RNA-seq.