Schwann Cell Tn5Prime

Here we provide sequencing data derived from our efforts to identify SOX10-regulated promoters in Schwann cells genome-wide. We assess the activity of SOX10-bound promoters using Tn5Prime to identify and quantify transcription start sites in adult peripheral nerve, in differentiating primary Schwann cells, and upon ablation of SOX10 in vitro. Tn5Prime libraries were prepared essentially as described by Cole and colleagues (NAR, 2018). Analyses focused on transcription start sites associated with H3K4me3 and SOX10 ChIP-Seq peaks from sciatic nerve (ChIP-Seq data derived from previously published datasets). Samples include two libraries derived from independent adult rat sciatic nerves (age 6-9 months), three libraries each from control- and CPT-cAMP-treated rat primary Schwann cells, two libraries from unmodified immortalized rat myelinating Schwann (S16) cells, and four libraries each derived from independent clonal ΔSOX10 S16 cell lines. Each library was prepared from an independent RNA isolation.