RNA-sequencing of murine B16F10 melanoma cells with IFNg and FK866, a Nampt inhibitor

NAMPT is expressed in all cells during normal cell homeostasis. However, melanoma cells have a higher energetic demand and increase NAMPT expression. Our data show that IFNg signaling upregulates NAMPT in both human and mouse melanoma cells leading to increased cell growth and expression kinetics. The purpose of this experiment was to identify how IFNg inducible Nampt alters cell kinetics and we did this by treating melanoma cells with the combination of IFNg and FK866. B16F10 murine melanoma cells were treated with IFNg (50ng/mL) and FK866 (50nM) for 6 hours and then processed for RNA-sequencing via 10x platform.