Mutant FUS and ELAVL4 (HuD) aberrant crosstalk in Amyotrophic Lateral Sclerosis. Mutant FUS and ELAVL4 (HuD) aberrant crosstalk in Amyotrophic Lateral Sclerosis

Determination of RNAs bound by wildtype and P525L mutant FUS in human iPSC-derived motoneurons using PAR-CLIP (Photoactivatable Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation) Overall design: First, endogeneous FUS was immunoprecipitated from wildtype and FUS-P525L mutant motoneurons. Second, FUS wildtype or the P525L mutant was ectopically expressed as a flag-fusion protein and immunoprecipitated using an anti-flag antibody.