Gene expression profiling in prostate cancer cell line LNCaP.

Global definition of androgen and anti-androgen effects on LNCaP transcriptomes using Affymetrix U133A oligonucleotide microarray. LNCaP in androgen-depleted medium was treated with androgen (DHT) and anti-androgen (CPA) for different time points (2 hours, 4 hours, 8 hours, and 24 hours). Untreated or Vehicle (Ethanol) treated samples as control. Total RNA was extracted from duplicate LNCaP samples using Trizol reagent. Biotin-labeled cRNA was generated and hybridized to Affymetrix U133A oligonucleotide microarrays as described (PMID: 10521349). The expression files were normalized across the arrays, and genes expressed below 10 were filtered out. For each gene, the average expression level in the control samples (no treatment or Vehicle-treated) was set as 1.0, and expression at each time point in the treated samples was expressed as fold change relative to this value. Submitter states that raw data files are not available.