ISGC Sheep Genome Sequences

The collection contains the genome sequence of a diverse collection of sheep. For each animal, sequence reads are available to a depth of approximately 10 fold genome coverage. Sequence reads are made available in BAM format. Sequence polymorphisms are made available in VCF format following comparison of each animal’s sequence to the sheep reference genome assembly (v3.1). To assist users with interpretation of the collection, the reference genome (FA format) and genome feature files (GFF3 format) are included for viewing using software such as the Integrative Genome Viewer (IGV).\nLineage: Sequence reads of each animal were generated using Illumina sequencing of short (200 – 300 bp) insert libraries. Alignment for sequence reads was performed against the sheep reference genome assembly (v3.1) using bwa to create BAM files. Aligned reads were used for variant detection using two independent pipelines; AtlasSNP and SamTools. Only variants independently identified using both pipelines were made available.