Effects of PAK4 knockdown on gene expression in glioblastoma-associated endothelial cells
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE154133
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资源简介：
PAK4 was knocked down in human glioblastoma (GBM)-associated endothelial cells (ECs) using CRISPR-Cas9/gRNA. RNA was isolated and analyzed by RNAseq. The effects of PAK4 knockout on gene expression were determined. ECs were isolated from GBM tumors and transduced with the lentivirus expressing PAK4 sgRNA or control sgRNA, followed by cell selection by incubation with medium containing 2 µg/ml puromycin. RNA was isolated and analyzed by RNAseq.
创建时间：
2023-05-11
