Ip, Wallace (2010) CIL:6561, Oryctolagus cuniculus, skeletal muscle cell. CIL. Dataset

Rabbit psoas skeletal muscle fibers were held at constant length and rigor induced by immersion in glycerol. The fibers were fixed in buffered glutaraldehyde, treated with tannic acid and quick-frozen by contact with a liquid helium-cooled copper block in a Heuser-type cryopress, and freeze-etched in a Balzers 400 freeze fracture machine. The sample was then rotary-replicated with Pt-C at 24 degrees and visualized in a JEOL 100CX2 transmission electron microscope operated at 100 kev.

This image was recorded on Kodak 4489 film at 75,000x magnification. Stereo pairs were taken at +5 and -5 degrees. Films were scanned with a 20 micrometer pixel spacing on a Nikon Coolscan 9000ED scanner.

Stereo pair with CIL:6565.

本研究中，以甘油沉浸法维持恒定长度的兔psoas骨骼肌纤维，经甘油溶液处理后引发僵直。随后，肌纤维经缓冲戊二醛固定，经鞣酸处理，并通过与液氦冷却的铜块接触在Heuser型冷冻压机中快速冷冻，并在Balzers 400冷冻断裂机上进行了冷冻蚀刻。样品随后在24度角下以Pt-C进行旋转复制，并在运行于100 kev的JEOL 100CX2透射电子显微镜下进行可视化观察。图像记录于柯达4489胶片上，放大倍数为75,000倍。在+5度和-5度处采集了立体对图像。胶片以20微米像素间距在尼康Coolscan 9000ED扫描仪上进行了扫描。CIL:6565对应的立体对图像亦被采集。