Ashwagandha: optimizing the extraction and electrospun nanofiber production

Dissolution.xlsx - data represent the percentage of withanoside IV released. Columns indicate the systems tested, rows the corresponding time points. Data are presented as µg. Dissolution studies of electrospun nanofibers were performed using an Agilent 708-DS dissolution apparatus. A standard basket method was used at 37 ± 0.5 °C with a stirring speed of 50 rpm. Nanofibers were placed in 300 mL of artificial saliva solution at pH 6.8 (potassium chloride (1.20 g), sodium chloride (0.85 g), di-potassium hydrogen orthophosphate (0.35 g), magnesium chloride (0.05 g), calcium chloride (0.20 g), xylitol (20.0 g) and water up to 1 L; pH was adjusted to 6.8 by 1 M HCl). The liquid samples were collected at specified time intervals, and an equal volume of temperature-equilibrated media was replaced. The samples were filtered through a 0.45 μm nylon membrane filter. The content was determined using the HPLC method (https://www.mdpi.com/1999-4923/17/1/61).