Sustained down-regulation of alphaSmooth muscle actin expression in aortas induced by angiotensin II: a new model of vascular memory to hypertension

We developped a new model of vascular injury memory in response to AngII stimulation in mice. We used RNAseq (aortas) to try to understand observed sustained phentoype and identify Acta2 (aSMA) as key in observed phenomenon. Overall design: 11-week male C57B6 mice were implanted with osmotic minipumps delivering of 2.8mg/kg/day of AngII for 2 weeks. Mice were sacrificed at the end of the treatment (AngII); and after 2 and 3 weeks of AngII (AngII+2W; Ang+3W, respectively). Age matched littermate served as control. Aortic mRNA profile was compared: CTRL vs AngII; CTRL vs AngII+2W; and CTRL vs AngII+3W